Gene Transfer Mechanism in Bacteria. The mechanism resembles bacterial conjugation and the “virulence” genes of the Ti-plasmid are equivalent to the tra genes of other plasmids. Three broad mechanisms mediate efficient movement of DNA between cells- conjugation, transduction and transformation. Bacterial conjugation is a promiscuous DNA transport mechanism. The prototypical conjugative plasmid is the F-plasmid, or F-factor. Physical Contact between Donor and Recipient Cell. Conjugation: Transfer of genes between cells that are in physical contact with one another; Transduction: Transfer of genes from one cell to another by a bacteriophage The donor cells (F+ cells) form a sex pilus and begin contact with an F- recipient cell. This tube formed between both cells (i.e., the donor and recipient bacterial cell) is known as sex pilus or just pilus. Bacterial conjugation is the transfer of genetic material between bacterial cells by direct cell-to-cell contact or by a bridge-like connection between two cells. Bacterial conjugation is a genetic transfer that involves cell‐to‐cell between donor and recipient cells. Bacterial conjugation is a way by which a bacterial cell transfers genetic material to another bacterial cell. MECHANISM OF CONJUGATION. Conjugation is the process by which one bacterium transfers genetic material to another through direct contact. Mechanism. Mechanism of Bacterial Conjugation. With the current method used to teach students in genetic courses at the undergraduate level, the transconjugants are identified using bacterial physiology and/or antibiotic resistance. In conjugation, DNA is transferred between bacteria through a tube between cells. The F-plasmid is an episome (a plasmid that can integrate itself into the bacterial chromosome by homologous recombination) with a length of about 100 kb. Conjugative plasmids transfer themselves between most bacteria, thus being one of the main causal agents of the spread of antibiotic resistance among pathogenic bacteria. Bacterial conjugation involves the following steps: Pilus Formation. The T-DNA is covered by the VirE2 protein made by Agrobacterium and also, when it enters the plant cell, by the plant VIP1 protein. Bacterial Conjugation - Mechanism. The genetic material that is transferred through bacterial conjugation is a small plasmid, known as F-plasmid (F for fertility factor), that carries genetic information different from that which is already present in the chromosomes of the bacterial cell. 7/23/2016 3 4. The pilus forms a conjugation tube and enables direct contact between the donor and the recipient cells. Moreover, DNA can … Bacterial Conjugation Definition.
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