The lab has recently isolated two compounds that bind directly to and act as inhibitors of TspZ; these inhibitors have been named Inh41 and Inh42. If you want the Solutions Manual please search on the search box. Both Inh41 and Inh42 seem to reduce amino acid transport 10-fold when added to cells individually. A test bank will include the following questions: Membrane Transport of Small Molecules and the Electrical Properties of Membranes, Molecular Biology of the Cell, Fifth Edition. This mutation locked the protein into a particular conformation, so that the transporter was inward-facing such that its sugar-binding domain faced the cytoplasmic portion of the cell. The equilibrium potential will be negative for K+ ions and positive for Na+ ions. Download Molecular Biology Of The Cell Question Bank free PDF at Info-Nexus. 1.2 Ultrastructure of cells. More about Molecular Biology Of The Cell Question Bank in portable document format (PDF) you can enjoy and download here This is because the delayed K+ channels are normally involved in the repolarization of the cell. Full file at Since Inh31 and Inh42 probably interact with different parts of TspZ, adding both inhibitors will probably have a greater effect of decreasing amino acid uptake than will the addition of either inhibitor alone. In contrast, Inh42 is a competitive inhibitor, which implies that the mechanism by which Inh42 works involves its competing for the binding site of TspZ with amino acids. As a result, the web page can not be displayed. An Error 522 means that the request was able to connect to your web server, but that the request didn't finish. Mg2+ is a charged ion and therefore does not interact well with the hydrophobic hydrocarbon portion of the lipid bilayer. If the normal, unmutated version of the protein were used for crystallization studies, different conformational states of the protein might have existed and hindered the crystallization process, which requires the formation of well-ordered crystals containing many copies of a protein in a particular conformation to collect analyzable x-ray diffraction data. 11-5     You would predict that the addition of Inh41 and Inh42 to cells might have an effect greater than adding either alone. The conformation obtained often reflects the lowest free-energy form of the protein. The movement of ions will create a chemical gradient, but not an electrical gradient, because you are swapping a positive divalent ion for another positive divalent ion. Our library is the biggest of these that have literally hundreds of thousands of different products represented. The opening of chloride channels will make it harder for the cell to fire an action potential. This question bank and associated answer sheet were compiled by Yilun Du and Varun Mangalick for. Specifically, be sure to state whether you think there would be net movement of ions and if so, how the concentrations of the Mg2+ and Ca2+ ions inside and outside the bilayer would change. Molecular Biology of the Cell 6th Edition Bruce Test Bank with answer keys for the tests question only NO Solutions for Textbook's Question included on this purchase. The opening of chloride channels will cause chloride to move into the cell, further reducing the resting potential of the neuron. Explain your answer. View Test Prep - Test-bank-for-Molecular-Biology-of-the-Cell-6th-edition-by-Bruce-Alberts.doc from SCIENCE 2015 at University of Texas. Using the C154G mutation that strongly favors the formation of a single conformation facilitated the formation of well-diffracting crystals that allowed the structure to be determined. 11-5     Your friend works in a lab that is interested in the properties of an amino acid transporter called TspZ. If a normal cell produces a pattern of membrane depolarization following the combined PSPs illustrated in Figure Q11-8, how would a drug that reduces the activity of the delayed K+ channels alter the pattern of membrane depolarization? 11-8     The action potential will last longer (the peak will be broader) because inhibition of the delayed K+ channels will inhibit repolarization of the neuron. You produce lipid vesicles that contain this Mg2+–Ca2+ pump as the sole membrane protein. 1.4 … He thinks his data support the idea that a protein is required for the uptake of his drug; his supervisor thinks his data show that this drug simply diffuses into the cell. Contact us and submit your document if you believe any copyright infringement has occurred. 11-6     Recall that the Nernst equation is, For univalent ions at 20°C, the equation reduces to. Although O2 is indeed larger than Mg2+, because it is a nonpolar molecule it can diffuse more rapidly than Mg2+ across a lipid bilayer. 11-3     Your friend works for a biotech company, where they have come up with a top- secret drug they hope will cure a hereditary kidney disease. Your friend’s experiments show that Inh41 acts as a noncompetitive inhibitor of TspZ, because Inh41 addition decreases Vmax (the rate at which TspZ can flip between two conformational states) and does not seem to affect Km (the affinity of TspZ for amino acids), suggesting that Inh41 does not bind TspZ at its amino acid binding site. a) being exothermic The Mg2+–Ca2+ pumps are oriented such that Mg2+ is pumped into the vesicle while Ca2+ is pumped out. This means that more acetylcholine-gated cation channels must open to reach the amount of depolarization required to open the mutant voltage-gated Na+ channel. Cloudflare Ray ID: 5f8dbb590f230560 Explain your answer. An Error 522 means that the request was able to connect to your web server, but that the request didn't finish. Cell biology. Molecular Biology of the Cell, Fifth Edition. The initial connection between Cloudflare's network and the origin web server timed out. All contents are provided by non-affiliated third parties. Get the latest updates on new products and upcoming sales, Copyright © 2020 | All rights reserved. Additional troubleshooting information here. The ion movement will not significantly change the concentrations outside the vesicle. Contact your hosting provider letting them know your web server is not completing requests. For transporter-mediated diffusion, the rate of update by the cell will eventually saturate as you increase the concentration of drug, depending on how many protein molecules there are at the membrane to take in the molecule. By inhibiting the delayed K+ channels, the cell will take longer to return to its resting membrane potential, and that is why the peak of the action potential will be broader. Explain your answer. The concentration of Ca2+ ions within the vesicle will decrease and the concentration of Mg2+ ions within the vesicle will increase. The most likely cause is that something on your server is hogging resources. What student Can Expect From A Test Bank? Explain your answer. For additional problem banks/tutoring for USABO contact either Yilun Du at or Varun Mangalick at • To characterize how Inh41 works, your friend has discovered that Inh41 does not affect the ability of TspZ to bind amino acid, but instead seems to decrease the rate at which TspZ is able to change its conformation. 1.1 Introduction to cells. He refuses to tell you anything about this drug, except to give you a peek at their data (see Figure Q11-3) regarding the kinetics of uptake of this drug into kidney cells.

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